Antibody FREE method to capture 5-hmC Methylation
Active Motif's Hydroxymethyl Collector™ Kit is designed to detect and capture DNA fragments containing 5-hydroxymethylcytosine (5-hmC) DNA methylation. The Hydroxymethyl Collector kit utilizes a β-glucosyltransferase enzyme to transfer a modified glucose moiety to 5-hydroxymethylcytosine residues in double-stranded DNA. This modified glucose is then used to chemically attach a biotin conjugate for capture and enrichment with streptavidin magnetic beads.
For more information about Hydroxymethyl Collector, click here.

Selective chemical labeling reveals the genome-wide distribution of 5-hydroxymethylcytosine
The group at the University of Chicago used the T4 bacteriophage β-glucosyltransferase to transfer an engineered glucose moiety containing an azide group onto the...view publication.
Song et al. Nat. Biotechnol. 2011. 29(1): 68-72

Features

• Minimum 5 ng DNA/sample
• More stringent than antibody detection
• No cross-reactivity with 5-mC
• Pulls down CpG, CpA and CpT methylation
• Not constrained by glucosyl-sensitive restriction enzymes
• Complete in less than 4 hours

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Hydroxymethyl Collector Kit for enrichment of 5-hmC DNA.
Mouse brain DNA was assayed in the Hydroxymethyl Collector Kit at various quantities. Enriched DNA was analyzed by qPCR across multiple loci. The enriched DNA was plotted as a percentage of starting material. This graph shows that Hydroxymethyl Collector has a preferred range of detection from 5 ng to 2.5 µg.

Bisulfite Conversion

DNMT Activity/Inhibition

Methylated DNA Standards

PvuRts1 I restriction enzyme

CpG Enrichment

MeDIP

DNA Methylation Antibodies

Whole Genome Amplification

CXXC Enrichment

hMeDIP

β-Glucosyltransferase enzyme

DNA Methylation Services