Why do you need Active Motif's new 5-hmC antibody?

• It discriminates 5-hmC from 5-mC; only 5-hmC is recognized
• Enrichment by existing 5-mC antibodies doesn't work on 5-hmC
• Enzymatic approaches and bisulfite sequencing cannot differentiate 5-mC from 5-hmC in DNA
• Methyl-CpG DNA binding proteins do not recognize 5-hmC

MeDIP analysis of 5-hmC antibody

DNA (25 pg) derived from the promoter of the APC gene was spiked into 500 ng of human genomic DNA and subjected to the MeDIP procedure using 1 μl of 5-Hydroxymethylcytidine antibody (5hmC, purple bars) or 1 μl of control rabbit IgG (IgG, copper bars). Real time quantitative PCR was performed on the immunoprecipitated DNA and results plotted as % of input DNA. The spiked APC DNA contained either no methylation (DNA), 5-methylcytosine methylation (5-mC) or 5-hydr

Dot blot analysis of 5-hmC antibody

Panel A: 5-Hydroxymethylcytidine antibody (1:10,000 dilution). Panel B: 5-Methylcytidine antibody (1:1,000 dilution). Lane 1: DNA derived from mouse embryonic stem cells (150 ng). Lane 2: DNA derived from mouse spleen (600 ng). Lane 3: 27 base oligonucleotide containing 5-Hydroxymethylcytosine (1.2 ng). Lane 3: 24 base oligonucleotide containing 5-methylcytosine (2000 ng).