Epigenetics News and New Antibody Products

Epigenetics News

Histone demethylase linked to X-linked mental retardation
Working in the model organism zebrafish, researchers at Harvard University have discovered a role for the histone demethylase PH8 in craniofacial and brain development. Ablation... more.

Reprogrammed stem cells retain hallmarks of the original cell type
Two recent papers about induced pluripotent stem cells (iPSCs) describe findings that indicate that these cells retain cell fate programs that are similar to the cell type from which... more.

Tet1 protein plays an important role in stem cell pluripotency
A study conducted at the University of North Carolina at Chapel Hill concludes that the Tet1 enzyme is a key regulator of stem cell pluripotency and developmental fate... more.

Product Guides

Antibodies

Chromatin IP

Chromatin Biology & Epigenetics

Transcription

New Antibodies

AIB3 pAb (Catalog No. 39835) details
Applications: IHC, IP, WB

AIB3 (Amplified in Breast Cancer 3, Nuclear Receptor Coactivator 6, NCoA6) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB3 / NCoA6 was originally isolated as a ligand-dependent nuclear receptor interacting protein. It is a co-activator that directly binds nuclear receptors and stimulates their transcriptional activity in a hormone-dependent fashion. AIB3 is a multifunctional co-activator necessary for transcriptional activation of a wide spectrum of target genes. AIB3 is involved in growth, development, wound healing and maintenance of energy homeostasis. It can enhance the transcriptional activation of a variety of transcription factors and also associates with a number of important regulators of transcription including CBP and p300. AIB3 / NCoA6 is a component of at least three distinct MLL complexes, suggesting that AIB3 plays a fundamental role in transcriptional activation by modulating chromatin structure through histone methylation. AIB3 may also be involved in the co-activation of the NF-kappa-B pathway. It is amplified and overexpressed in breast, colon and lung cancers. AIB3 function is essential in mammalian development as AIB3 null embryos do not survive. Additionally, AIB null spontaneously undergo apoptosis, indicating that AIB3 has anti-apoptotic and pro-survival functions.

Histone H3 phospho Thr11 mAb (Catalog No. 39821) details
Clone: N123/48
Applications: ICC, WB

Histone H3 is one of the core components of the nucleosome, the basic subunit of chromatin. Histone H3 phosphorylation occurs at several sites, including Thr11, and these modifications are tightly correlated with chromosome condensation during both mitosis and meiosis.

HNF-3a / FOXA1 mAb (Catalog No. 39837) details
Applications: IHC, WB

HNF-3α (Hepatocyte nuclear factor 3 alpha, forkhead box A1, FoxA1) is a homeodomain containing transcription factor and is a member of the Hepatocyte Nuclear Factor family (HNF-1α, HNF-1β, HNF-3α, HNF-3β, HNF-3γ, HNF-4α, HNF-4γ, HNF-6α and HNF-6β), whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3α and HNF-3γ have also been shown to be involved in events such as gastrulation and body axis formation. HNF-3α modulates transcriptional activity of the estrogen receptor and is involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate.

HNF-3b / FOXA2 pAb (Catalog No. 39827) details
Applications: IHC, WB

HNF-3β (Hepatocyte nuclear factor 3 beta, forkhead box A2, FoxA2) is a homeodomain containing transcription factor and a member of the Hepatocyte Nuclear Factor family whose members regulate the transcription of numerous hepatocyte genes in adult liver. HNF-3 members bind to DNA as monomers using a winged helix DNA-binding domain. HNF-3β is involved in glucose homeostasis and fat metabolism. It regulates the expression of genes important for glucose sensing in pancreatic beta-cells.

MPP8 mAb (Catalog No. 39819) details
Clone: N119/44
Applications: ICC, WB

MPP8 (M-Phase Phosphoprotein 8) was originally identified as a phosphoprotein that reacts with MPM2, an antibody that labels mitosis-specific and cell cycle-regulated phosphoproteins. MPP8 contains a single chromodomain that preferentially interacts with nucleosomes enriched for histone H3 lysine 9 methylation but not lysine 4 methylation.

NAP1L1 pAb (Catalog No. 39841) details
Applications: WB

NAP1L1 (Nucleosome assembly protein 1-like 1; NAP1-Related Protein, NRP) is a member of the NAP family of histone chaperones. Histone chaperones are involved in chromatin assembly following DNA replication.

SATB1 pAb (Catalog No. 39839) details
Applications: WB

SATB1 (Special AT-rich sequence-binding protein 1) is a chromosome-associated protein that binds to matrix attachment regions (MARs) of DNA at special AT-rich sequences and induces local chromatin-loop remodeling. SATB1 functions as both a chromatin organizer and transcription factor. It regulates gene expression and higher-order chromatin structure of multigene clusters. SATB1 acts as a docking site for several chromatin remodeling enzymes and recruits corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. It is required for the switching of fetal globin genes, plays a role in chromatin organization and nuclear architecture during apoptosis. It is a crucial factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis. SATB1 contributes to metastatic potential in breast cancer cells, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. It is also required for the differentiation of embryonic stem cells through repression of ES pluripotency factors Nanog, Klf4, and Tbx3.

SMRT / NCoR2 pAb (Catalog No. 39845) details
Applications: WB

SMRT / NCoR2 (Silencing Mediator of Retinoic acid and Thyroid hormone receptor, Nuclear receptor RoRepressor 2, SMAP270, CTG26) is a co-repressor of transcription that associates with a subset of nuclear receptors including RAR, PR, GR and TR in the absence of ligand. It is part of a large co-repressor protein complex that includes SIN3A/B and histone deacetylases HDAC1 and HDAC2.

Sox2 pAb (Catalog No. 39843) details
Applications: WB

Sox2 (SRY related HMG BOX gene 2) is a DNA binding transcription factor and a member of the SOX family of proteins, transcription factors that have an HMG type DNA binding motif. Sox2 forms a complex with Oct4 and controls the expression of a number of genes involved in embryonic. Sox2 is critical for early embryogenesis and required for embryonic stem cell pluripotency and thus can serve as a marker of stem cells. Overexpression of Sox2 (along with Oct4, KLF4 and c-Myc) can transform mouse fibroblasts into a state resembling embryonic stem cells (ES cells), referred to as Induced Pluripotency. Defects in Sox2 are the cause of microphthalmia syndrome type 3.