Epigenetics News and New Antibody Products

Epigenetics News

Organization of Genomic DNA in Loops linked to Transcriptional Regulation
Researchers led by a team at MIT and The Whitehead Institute have identified a mechanism that contributes to the higher order organization of chromatin and relates it directlyi...more.

Mapping the Methylome During Myeloid and Lymphoid Cell Development
In order to build a greater understanding of the process of cellular differentiation and the epigenetic events involved in the lineage restriction of pluripotent progenitor cells, researchers...more.

Histone Signature of Meiosis Found to be Conserved from Yeast to Mammals
Working initially in budding yeast, a team of researchers at the University at Pennsylvania uncovered a series of histone modifications required for meiosis and spore formation...more.

Product Guides

Antibodies

Chromatin IP

Chromatin Biology & Epigenetics

Transcription

New Antibodies

Ago2 mAb (Catalog No. 39853) details
Clone: 1B1-E2H5
Applications: IF, IP, WB

Argonaute 2 (Ago2, aka EIF2C2) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage.

Ago4 mAb (Catalog No. 39855) details
Clone: 1B7-G11
Applications: IP, WB

Argonaute 4 (Ago4, aka EIF2C4) is a member of the Argonaute family of proteins involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Ago4 lacks RNase activity and does not appear to cleave target mRNA molecules. It binds to microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Ago4 has both a PAZ and a PIWI domain, indicative of the Argonaute family of proteins.

BORIS / CTCFL pAb (Catalog No. 39851) details
Applications: WB

BORIS (Brother of Regulator of Imprinted Sites) / CTCFL (CTCF-like) is a zinc-finger DNA binding protein that acts as a chromatin insulator and a paralog of the CTCF protein. Normally, BORIS / CTCFL is expressed only in primary spermatocytes and is thought to play a role in resetting DNA methylation marks during the differentiation of male germ cells. BORIS / CTCFL is also expressed in many cancers and may serve a pro-proliferative function.

CTBP pAb (Catalog No. 39847) details
Applications: WB

CTBP (C-terminal Binding Protein, CTBP1) is a transcriptional repressor that was originally identified by virtue of its ability to bind the C-terminus of E1A proteins. CTBP serves as a co-repressor for a number of DNA binding transcription factors. CTBP is part of a variety of co-repressor complexes, many of which include HDAC proteins. CTBP also interacts with the vertebrate Polycomb protein hPc2, suggesting that repression of many CTBP-regulated genes also involves recruitment of the Polycomb complex.

MBD1 pAb (Catalog No. 39857) details
Applications: WB

MBD1 (methyl-CpG binding domain protein 1) functions as a mediator of the biological consequences of DNA methylation. Generally associated with the repressive chromatin state. methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as development and the control of gene expression. The complex series of events leading to this repressive state involve the coordinated regulation of DNA methyltransferases and two other groups of proteins called the Methyl-CpG binding proteins (MBD proteins) and the Kaiso family of proteins. The MBD family of proteins includes MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD1 is recruited to both methylated and non-methylated CpGs via separate domains: the CXXC domain targets the protein to non-methylated CpGs whereas the MBD domain targets the protein to methylated CpG.

Nucleophosmin pAb (Catalog No. 39849) details
Applications: WB

Nucleophosmin (NPM, NPM1, Nucleolar phosphoprotein B23) is a highly phosphorylated nucleolar protein involved in ribosome biogenesis, maintenance of genome stability, is directly implicated in cancer pathogenesis and serves as a histone chaperone. Nucleophosmin is closely related to Nucleoplasmin and shuttles back and forth between the nucleolus and the nucleoplasm. Chromosomal aberrations involving Nucleophosmin were found in patients with non-Hodgkin lymphoma, acute promyelocytic leukemia, myelodysplastic syndrome, and acute myelogenous leukemia. Nucleophosmin has been shown to interact with Nucleolin, AKT1, BRCA1 and BARD1. Mice deficient for Nucleophosmin have severe defects in genome stability due to unrestricted centrosome duplication. Heterozygous Nucleophosmin mutant mice show signs of myelodysplastic syndrome.

PGC1-β pAb (Catalog No. 39859) details
Applications: WB

PGC1-β (Peroxisome proliferator-activated receptor gamma coactivator 1-beta, PPARGC1B, PERC) is a co-activator of transcription by nuclear receptors and transcription factors. PGC1-β participates in modulating the transcriptional activity of PPAR alpha and gamma, ER alpha, NRF1 and GR. It is likely involved in fat oxidation, non-oxidative glucose metabolism and in the regulation of energy expenditure. Expression of PGC1-β is repressed by saturated fatty acids in skeletal muscle cells and induced by insulin. PGC1-β is down-regulated in type 2 diabetes as well as in pre-diabetics. Allelic variation of PGC1-β may contribute to the pathogenesis of obesity, with the widespread Ala-203 variation being a significant risk factor for the development of this diabetes.