Epigenetics News and New Antibody Products

Epigenetics News

Changes in Histone Abundance and Modification Distribution Observed During Aging
Cellular senescence, the process by which normal diploid cells lose the ability to divide, has long been studied as a model to examine organismal aging. While investigating telomere biology and potential...more.

Gene Specific Epigenetics Silencing Mechanism Found
Researchers at the German Cancer Research Center have identified a mechanism by which specific genes are selectively targeted for epigenetic silencing. The group identified a mechanism by which...more.

Link Uncovered Between DNA Hypomethylation and Multiple Myeloma
A research study conducted jointly at The Mayo Clinic and The Translational Genomics Research Institute has led to the finding that a global reduction in levels of DNA methylation is associated with the...more.

Free Histone Modification Poster

Active Motif announces the availability of our new Histone Modifications Guide, a poster filled with information on histone modifications, the enzymes that deposit and remove the marks, as well as the proteins that recognize modified histones. Request your copy today!

New Antibodies

Ago1/2/3 mAb (Catalog No. 39937) details
Clone: 2A8
Applications: ChIP, IF, IP, IHC, HITS-CLIP, RIP-ChIP, WB

The Argonaute family of proteins (Ago1, Ago2, Ago3 and Ago4) are involved in RNAi mediated gene silencing through siRNA and miRNA effectors. The Argonaute proteins are part of RISC, the RNAi Induced Silencing Complex. Binding of RISC can inhibit mRNA translation and results in the degradation of the mRNA through the action of Ago2-mediated target cleavage. Ago1 is non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage. Ago3 lacks RNase activity and does not appear to cleave target mRNA molecules. Argonaute family proteins contain a PAZ and a PIWI domain.

BRD4 pAb (Catalog No. 39909) details
Applications: WB

BRD4 (Bromodomain-containing protein 4, aka HUNK1) is a chromatin-associated protein with two bromo domains that binds acetylated histone H3 and H4. BRD4 is homologous to Xenopus MCAP, and like MCAP, BRD4 associates with chromosomes during mitosis, predominantly in the non-centromeric regions. A chromosomal aberration involving BRD4 (producing a BRD4-NUT fusion protein) is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. BRD4 is involved in the expression of extracellular matrix gene expression, and its misregulation may be involved in breast cancer progression.

DNMT1 pAb (Catalog No. 39905) details
Applications: WB

Methylation of mammalian DNA has long been recognized to play a major role in a number of cellular functions such as embryonic development, genetic imprinting, X chromosome inactivation and the control of gene expression. DNMT1 is the most abundant DNMT in somatic cells and shows a preference for methylating hemi-methylated DNA. It is considered to be a maintenance DNA methyltransferase that is important in the maintenance of specific patterns of methylation throughout cellular divisions.

DNMT3L pAb (Catalog No. 39907) details
Applications: WB

DNMT3L (DNA methyltransferase 3-like) is a nuclear protein with similarity to the DNMT family of proteins, but does not function as a DNA methyltransferase. It serves as an essential regulator of DNMT3A and DNMT3B, recruiting them to sites for deposition of de novo DNA methylation. DNMT3L is expressed in the presumptive gametes in mammalian embryos and is required for the establishment of genomic imprinting. DNMT3L binds to chromatin through interactions with the amino terminal tail of histone H3 that is devoid of lysine 4 methylation.

DOT1L pAb (Catalog No. 39953) details
Applications: WB

DOT1L (Disruptor of Telomeric silencing 1-like, also known as KMT4) is the mammalian homologue of the yeast Dot1 protein and the Drosophila Grappa protein. DOTL1 is the enzyme responsible for lysine 79 methylation of histone H3. DOT1L can promote an oncogenic pattern of gene expression through binding with several MLL fusion partners found in acute leukemia, thus leading to aberrant methylation of lysine 79 at MLL target genes. In contrast to most other histone lysine methyltransferases, DOT1L does not contain a SET domain. Biochemical studies suggest that recombinant DOT1L is active on recombinant nucleosomes.

ELP3 pmAb (Catalog No. 39949) details
Applications: WB

ELP3 (Elongator Protein 3, also called KAT9) was originally identified as a component of the RNA polymerase II elongator complex, involved in transcriptional elongation by RNA Pol II. Elongator, through ELP3 may play a role in chromatin remodeling and is involved in acetylation of histones H3 and possibly H4. ELP3 may also play a role as a DNA demethylase in vertebrates, as it is important for paternal DNA demethylation.

EZH2 pAb (Catalog No. 39901) details
Applications: ChIP

EZH2 (Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27. Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure and silencing gene expression. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 may play a role in the development of the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

EZH2 pAb (Catalog No. 39933) details
Applications: WB

Maz mAb (Catalog No. 39935) details
Clone: 133
Applications: ChIP, EMSA, IF, IP, WB

Maz (Myc-associated zinc finger protein) functions as a transcription factor roles in both transcription initiation and termination. Maz binds to two sites within the c-Myc promoter, ME1a1 and ME1a2. It also binds to multiple G/C-rich sites within the promoter of the Sp1 family of transcription factors. Maz also partipates in skeletal muscle development through the activation of several skeletal muscle-specific genes. Maz has also been reported to drive tumor-specific expression of PPAR-gamma in breast cancer cells.

MGMT pAb (Catalog No. 39951) details
Applications: WB

MGMT (O-6 methylguanine DNA methyltransferase) is an enzyme involved in DNA damage repair. MGMT transfers methyl groups from O-6 methylguanine methylated DNA, and other methylated adducts, to a cysteine residue in itself, thus reversing DNA damage caused by alkylation. This is a suicide reaction as the enzyme is irreversibly inactivated. MGMT helps prevent DNA alkylation events from causing mutations. It also contributes to resistance to chemotherapy in cancer patients being treated with alkylating drugs.

SIRT6 pAb (Catalog No. 39911) details
Applications: WB

SIRT6 (Sir2 homologue 6) is an NAD+ dependant histone deacetylase (HDAC) related to SIRT1 and to the yeast Sir2 protein. SIRT6, originally identified as a tubulin deacetylase, it was recently found to be able to deacetylate histone H3 acetylated at lysine 9.

SREBP-1 mAb (Catalog No. 39939) details
Clone: IgG-2A4
Applications: IP, WB

SREBP-1 and SREBP-2 (Sterol regulatory element-binding protein 1 and 2) are members of the basic-helix-loop-helix-leucine zipper (bHLH-ZIP) family of transcription factors and are involved in the regulation of cholesterol homeostasis. SREBP-1 and SREBP-2 are normally membrane bound at the ER and nuclear envelope, but in sterol-depleted cells, the membrane-bound precursors are cleaved to generate a soluble isoforms that translocate to the nucleus. These active forms, which includes the bHLH-ZIP domain, activate transcription of genes involved in the uptake and synthesis of cholesterol.

SREBP-2 mAb (Catalog No. 39941) details
Clone: IgG-1C6
Applications: ChIP, IP, WB

To give you wider options, many antibodies previously offered as rabbit serum are now available in purified IgG formats.

Histone H2A (yeast) pAb (Catalog No. 39945) details
Applications: WB

Histone H2A.Z pAb (Catalog No. 39943) details
Applications: WB

Histone H2B (yeast) pAb (Catalog No. 39947) details
Applications: WB

Histone H3 dimethyl Lys4 pAb (Catalog No. 39913) details
Applications: WB

Histone H3 trimethyl Lys4 pAb (Catalog No. 39915) details
Applications: WB

Histone H3 acetyl Lys9 pAb (Catalog No. 39917) details
Applications: WB

Histone H3 monomethyl Lys9 pAb (Catalog No. 39887) details
Applications: WB

Histone H3 dimethyl Lys27 pAb (Catalog No. 39919) details
Applications: WB

Histone H3 monomethyl Lys79 pAb (Catalog No. 39921) details
Applications: WB

Histone H3 dimethyl Lys79 pAb (Catalog No. 39923) details
Applications: WB

Histone H4 pan-acetyl pAb (Catalog No. 39925) details
Applications: WB

Histone H4 acetyl Lys12 pAb (Catalog No. 39927) details
Applications: WB

Histone H4 acetyl Lys16 pAb (Catalog No. 39929) details
Applications: WB